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transcriptase prime scripttm rt  (TaKaRa)


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    Structured Review

    TaKaRa transcriptase prime scripttm rt
    Transcriptase Prime Scripttm Rt, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 26722 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/transcriptase+prime+scripttm+rt/PrimeScript+RT+Master+Mix/pm41828407-252-18-22
    Average 99 stars, based on 26722 article reviews
    transcriptase prime scripttm rt - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    Synthesized:

    Article Title: Uncovering the Potential Mechanisms of Ergothioneine in Neuroinflammation Through Network Pharmacology, Molecular Docking, Molecular Dynamics Simulation, and In Vitro Validation.
    Article Snippet: BV2 cells were seeded into 96-well plates and cultured in DMEM at 37 ◦C for 24 h. After being washed with PBS, cells were treated with or without LPS (1 μg/mL) in combination with graded concentrations of the test compound (0.01, 0.1, 0.5, 1, and 10 μM), and incubated for 24 h. At each time point, 10 μL of CCK-8 reagent was added to each well, followed by incubation at 37 ◦C for 4 h. Absorbance was subsequently measured at 490 nm using a Thermo microplate reader (Thermo Fisher Scientific, Waltham, MA, USA), with plates maintained on a constant-temperature shaker prior to reading. .. Total RNA was extracted from cell tissues using the TRIzol method, and cDNA was synthesized with the reverse transcriptase Prime ScriptTM RT (Takara Premix Ex TaqTM II RR036A, Dalian, China), followed by PCR amplification. mRNA expression was detected in real time using the SYBR Green PCR Kit (TaKaRa SYBR® Premix Ex TaqTM II, Dalian, China) and the CFX96TM system (Bio-Rad, Hercules, CA, USA). ..

    Reverse Transcription:

    Article Title: Uncovering the Potential Mechanisms of Ergothioneine in Neuroinflammation Through Network Pharmacology, Molecular Docking, Molecular Dynamics Simulation, and In Vitro Validation.
    Article Snippet: BV2 cells were seeded into 96-well plates and cultured in DMEM at 37 ◦C for 24 h. After being washed with PBS, cells were treated with or without LPS (1 μg/mL) in combination with graded concentrations of the test compound (0.01, 0.1, 0.5, 1, and 10 μM), and incubated for 24 h. At each time point, 10 μL of CCK-8 reagent was added to each well, followed by incubation at 37 ◦C for 4 h. Absorbance was subsequently measured at 490 nm using a Thermo microplate reader (Thermo Fisher Scientific, Waltham, MA, USA), with plates maintained on a constant-temperature shaker prior to reading. .. Total RNA was extracted from cell tissues using the TRIzol method, and cDNA was synthesized with the reverse transcriptase Prime ScriptTM RT (Takara Premix Ex TaqTM II RR036A, Dalian, China), followed by PCR amplification. mRNA expression was detected in real time using the SYBR Green PCR Kit (TaKaRa SYBR® Premix Ex TaqTM II, Dalian, China) and the CFX96TM system (Bio-Rad, Hercules, CA, USA). ..

    Polymerase Chain Reaction:

    Article Title: Uncovering the Potential Mechanisms of Ergothioneine in Neuroinflammation Through Network Pharmacology, Molecular Docking, Molecular Dynamics Simulation, and In Vitro Validation.
    Article Snippet: BV2 cells were seeded into 96-well plates and cultured in DMEM at 37 ◦C for 24 h. After being washed with PBS, cells were treated with or without LPS (1 μg/mL) in combination with graded concentrations of the test compound (0.01, 0.1, 0.5, 1, and 10 μM), and incubated for 24 h. At each time point, 10 μL of CCK-8 reagent was added to each well, followed by incubation at 37 ◦C for 4 h. Absorbance was subsequently measured at 490 nm using a Thermo microplate reader (Thermo Fisher Scientific, Waltham, MA, USA), with plates maintained on a constant-temperature shaker prior to reading. .. Total RNA was extracted from cell tissues using the TRIzol method, and cDNA was synthesized with the reverse transcriptase Prime ScriptTM RT (Takara Premix Ex TaqTM II RR036A, Dalian, China), followed by PCR amplification. mRNA expression was detected in real time using the SYBR Green PCR Kit (TaKaRa SYBR® Premix Ex TaqTM II, Dalian, China) and the CFX96TM system (Bio-Rad, Hercules, CA, USA). ..

    Amplification:

    Article Title: Uncovering the Potential Mechanisms of Ergothioneine in Neuroinflammation Through Network Pharmacology, Molecular Docking, Molecular Dynamics Simulation, and In Vitro Validation.
    Article Snippet: BV2 cells were seeded into 96-well plates and cultured in DMEM at 37 ◦C for 24 h. After being washed with PBS, cells were treated with or without LPS (1 μg/mL) in combination with graded concentrations of the test compound (0.01, 0.1, 0.5, 1, and 10 μM), and incubated for 24 h. At each time point, 10 μL of CCK-8 reagent was added to each well, followed by incubation at 37 ◦C for 4 h. Absorbance was subsequently measured at 490 nm using a Thermo microplate reader (Thermo Fisher Scientific, Waltham, MA, USA), with plates maintained on a constant-temperature shaker prior to reading. .. Total RNA was extracted from cell tissues using the TRIzol method, and cDNA was synthesized with the reverse transcriptase Prime ScriptTM RT (Takara Premix Ex TaqTM II RR036A, Dalian, China), followed by PCR amplification. mRNA expression was detected in real time using the SYBR Green PCR Kit (TaKaRa SYBR® Premix Ex TaqTM II, Dalian, China) and the CFX96TM system (Bio-Rad, Hercules, CA, USA). ..

    Expressing:

    Article Title: Uncovering the Potential Mechanisms of Ergothioneine in Neuroinflammation Through Network Pharmacology, Molecular Docking, Molecular Dynamics Simulation, and In Vitro Validation.
    Article Snippet: BV2 cells were seeded into 96-well plates and cultured in DMEM at 37 ◦C for 24 h. After being washed with PBS, cells were treated with or without LPS (1 μg/mL) in combination with graded concentrations of the test compound (0.01, 0.1, 0.5, 1, and 10 μM), and incubated for 24 h. At each time point, 10 μL of CCK-8 reagent was added to each well, followed by incubation at 37 ◦C for 4 h. Absorbance was subsequently measured at 490 nm using a Thermo microplate reader (Thermo Fisher Scientific, Waltham, MA, USA), with plates maintained on a constant-temperature shaker prior to reading. .. Total RNA was extracted from cell tissues using the TRIzol method, and cDNA was synthesized with the reverse transcriptase Prime ScriptTM RT (Takara Premix Ex TaqTM II RR036A, Dalian, China), followed by PCR amplification. mRNA expression was detected in real time using the SYBR Green PCR Kit (TaKaRa SYBR® Premix Ex TaqTM II, Dalian, China) and the CFX96TM system (Bio-Rad, Hercules, CA, USA). ..

    SYBR Green Assay:

    Article Title: Uncovering the Potential Mechanisms of Ergothioneine in Neuroinflammation Through Network Pharmacology, Molecular Docking, Molecular Dynamics Simulation, and In Vitro Validation.
    Article Snippet: BV2 cells were seeded into 96-well plates and cultured in DMEM at 37 ◦C for 24 h. After being washed with PBS, cells were treated with or without LPS (1 μg/mL) in combination with graded concentrations of the test compound (0.01, 0.1, 0.5, 1, and 10 μM), and incubated for 24 h. At each time point, 10 μL of CCK-8 reagent was added to each well, followed by incubation at 37 ◦C for 4 h. Absorbance was subsequently measured at 490 nm using a Thermo microplate reader (Thermo Fisher Scientific, Waltham, MA, USA), with plates maintained on a constant-temperature shaker prior to reading. .. Total RNA was extracted from cell tissues using the TRIzol method, and cDNA was synthesized with the reverse transcriptase Prime ScriptTM RT (Takara Premix Ex TaqTM II RR036A, Dalian, China), followed by PCR amplification. mRNA expression was detected in real time using the SYBR Green PCR Kit (TaKaRa SYBR® Premix Ex TaqTM II, Dalian, China) and the CFX96TM system (Bio-Rad, Hercules, CA, USA). ..



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